Cryo-SEM of Perpendicular Cross Freeze-Fractures Through a High-Pressure-frozen Biofilm
نویسندگان
چکیده
The cryo scanning electron microscopy (cryo-SEM) as well as high-pressure-freezing (HPF) belongs to cutting edge techniques in electron microscopy of hydrated samples [1]. However, their combination is not always easily applicable. Here, we present a way of combining high-pressure-freezing using EM PACT2 (Leica Microsystems) that fixes hydrated samples on a 1.4 mm sapphire discs and high resolution SEM JEOL 7401F equipped with the cryo-attachment ALTO 2500 (GATAN). The freeze-fracturing technique is used here for investigation of yeast cultures cultivated on the sapphire discs, where we focus on the formation of the extracellular matrix produced during the cultivation.
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